Alamar blue invitrogen manual

indicator that is blue in color and non-fluorescent. When incubated withviable cells, the reagent changes color from blue to red and becomes fluorescent (Figure 1). This change can be detected using fluorescence or absorbance measurement instruments (see Procedure Summary Section) (Figure 2 File Size: KB. alamarBlue®. alamarBlue ® indicator dye (BUFB) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring. Published customer image: alamarBlue™ (BUFA) used to assess cell viability of N2a cells following various treatments . Overview. alamarBlue is a cell viability assay reagent which contains the cell permeable, non-toxic, and weakly fluorescent blue indicator dye called resazurin. This is a trusted and established reagent which has been available since alamarBlue is a useful non-toxic alternative to the commonly used MTT cell viability assay (3- (4,5.

The Alamar Blue assay provides accurate time-course measurements, has high sensitivity and linearity, involves no cell lysis, is ideal for use with post-measurement functional assays, is flexible as it can be used with different cell models, is scalable and can be used with fluorescence- and/or absorbance-based instrumentation platforms, and finally, it is non-toxic, non-radioactive and is safe for the user, and the environment. indicator that is blue in color and non-fluorescent. When incubated withviable cells, the reagent changes color from blue to red and becomes fluorescent (Figure 1). This change can be detected using fluorescence or absorbance measurement instruments (see Procedure Summary Section) (Figure 2 and 3). Figure 1. alamarBlue®. alamarBlue ® indicator dye (BUFB) quantitatively measures proliferation in human, animal, bacterial, fungal and mycobacterial cells. It is useful for cytokine bioassays, cell proliferation studies and in-vitro cytotoxicity determinations as well as cell growth monitoring. Published customer image: alamarBlue™ (BUFA) used to assess cell viability of N2a cells following various treatments in vitro.

The fluorescence measured in those was used as a background and subtracted. However, although this protocol is routinely used in the literature, some variations. 12 Sept The AlamarBlue assay is based on fluorometric detection of metabolic mitochondrial activity of cells. In this study, we determined the. alamarBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of.